Article citationsMore >>

Huang, M.T., Lysz, T.T., Ferraro, T.F., Abidi, J.D., Laskin, A.H., 1991. Conney, Inhibitory effects of curcumin on in vitro lipoxygenase and cyclooxygenase activities in mouse epidermis, Cancer Res. 51, 813-819.

has been cited by the following article:

Article

Curcumin Suppresses the Activity of Inhibitor-κB Kinase in an in vitro Inflamed Human Intestinal Mucosa Model by S-nitrosylation

1Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan. R.O.C.


Journal of Food and Nutrition Research. 2017, Vol. 5 No. 6, 406-412
DOI: 10.12691/jfnr-5-6-7
Copyright © 2017 Science and Education Publishing

Cite this paper:
Ning-Jo Kao, Zwe-Ling Kong. Curcumin Suppresses the Activity of Inhibitor-κB Kinase in an in vitro Inflamed Human Intestinal Mucosa Model by S-nitrosylation. Journal of Food and Nutrition Research. 2017; 5(6):406-412. doi: 10.12691/jfnr-5-6-7.

Correspondence to: Zwe-Ling  Kong, Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan. R.O.C.. Email: kongzl@mail.ntou.edu.tw

Abstract

In previous study, we found curcumin to possess anti-inflammatory properties in lipopolysaccharide (LPS)-induced macrophage cells due to the involvement of curcumin and S-nitrosylation in the NF-κB pathway. However, the role of curcumin on regulation of NF-κB signaling pathway through S-nitrosylation in an in vitro inflamed human intestinal mucosa model has not yet been elucidated. This study aimed to concern inhibitory effects of curcumin on NF-κB pathway in two type of inflamed human intestinal cells, Caco-2 and HT-29. Western blot presented the protein expression of iNOS can be reduced by treated curcumin with 30 μM for 12h. Consistently, pro-inflammatory cytokines, such as IL-1β, IL-6, TNFα and IFN-γ was also repressed. The results also shows curcumin reduced the amount of nitrite and nitrate in inflamed human intestinal cells, Caco-2 and HT-29, maintained total S-nitrosylation level on proteins. Furthermore, the protection of S-nitrosylation on IKKβ in inflamed Caco-2 and HT-29 cells by curcumin caused the repression of IκB phosphorylation and NF-κB activation. In conclusion, this study verified that curcumin-mediated S-nitrosylation may be as an important regulator for anti-inflammation in an in vitro inflamed human intestinal mucosa model.

Keywords