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Ma, J.S., Kim, W.J., Kim, J.J., Kim, T.J., Ye, S.K., Song, M.D., Kang, H., Kim, D.W., Moon, W.K., and Lee, K.H., “Gold nanoparticles attenuate LPS-induced NO production through the inhibition of NF-jB and IFN-b/STAT1 pathways in RAW264.7 cells” Nitric Oxide 23(3), 214-219. 2010.

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Article

Anti-inflammatory Effects of Extract from Eucommia ulmoides Oliv. Leaves on Macrophage Cells

1Department of Korean Food & Culinary Arts, Youngsan University, Busan, 612-743, Korea


Journal of Food and Nutrition Research. 2017, Vol. 5 No. 5, 309-312
DOI: 10.12691/jfnr-5-5-4
Copyright © 2017 Science and Education Publishing

Cite this paper:
Hyun Woo Kang. Anti-inflammatory Effects of Extract from Eucommia ulmoides Oliv. Leaves on Macrophage Cells. Journal of Food and Nutrition Research. 2017; 5(5):309-312. doi: 10.12691/jfnr-5-5-4.

Correspondence to: Hyun  Woo Kang, Department of Korean Food & Culinary Arts, Youngsan University, Busan, 612-743, Korea. Email: khw7200@ysu.ac.kr

Abstract

This study investigated the anti-inflammatory effects of Eucommia ulmoides Oliv. (EUO) leaf extracts on RAW264.7 macrophages. Cell toxicity was determined by MTT assay. We evaluated the anti-inflammatory effects of EUO extracts by measuring nitric oxide (NO), inducible nitric oxide synthase (iNOS) production, and cyclooxygenase-2 (COX-2) expression by western blotting. EUO ethanolic extracts (0.1, 1, 5, and 10 μg/mL) significantly suppressed LPS-stimulated production of NO. EUO ethanolic extracts reduced the expression of iNOS and COX-2 proteins. The present results show that EUO ethanol extract has potent anti-inflammatory effects on RAW264.7 macrophages. These results also suggest that the anti-inflammatory effects of EUO extracts may be related to the inhibition of LPS-stimulated NO production. Therefore, ethanolic extracts of EUO leaves may be utilized as a good source of functional food and pharmaceutical applications for protection against inflammatory diseases.

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