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Article

Identification of Molecular Biomarkers for Food Quality Assessment of Oysters Exposed to Hypoxia

1South Sea Environment Research Department, Korea Institute of Ocean Science and Technology,Geoje,Republic of Korea

2Korea Marine Environment Management Corporation, Seoul, Republic of Korea

3Department of Life Science and Biotechnology, Shingyeong University, Hwaseong, Republic of Korea

4Department of Biological Science, Silla University, Busan, Republic of Korea


Journal of Food and Nutrition Research. 2017, Vol. 5 No. 3, 156-159
DOI: 10.12691/jfnr-5-3-3
Copyright © 2017 Science and Education Publishing

Cite this paper:
So Yun Park, Jinik Hwang, Juyun Lee, Youngjae Chung, Donggiun Kim, Taek-Kyun Lee. Identification of Molecular Biomarkers for Food Quality Assessment of Oysters Exposed to Hypoxia. Journal of Food and Nutrition Research. 2017; 5(3):156-159. doi: 10.12691/jfnr-5-3-3.

Correspondence to: Taek-Kyun  Lee, South Sea Environment Research Department, Korea Institute of Ocean Science and Technology,Geoje,Republic of Korea. Email: tklee@kiost.ac.kr

Abstract

Hypoxia, corresponding to a low oxygen concentration of less than 2.8 mg O2/L (91.4 mM), may cause serious problems in marine environments. In this study, we applied differential display PCR and fatty acid analysis to investigate molecular biomarkers for assessing hypoxic effects using the oyster, Crassostrea gigas, as a model organism. Oysters were exposed to normoxic (7.6 mg O2/L) or hypoxic (1.8 mg O2/L) concentrations of dissolved oxygen for 2 days. We found that glutamine synthetase (GS) gene expression decreased and glutathione S-transferase (GST) gene expression increased in oysters exposed to hypoxia. In addition, linoleic acid content significantly decreased following hypoxic exposure compared with controls. Collectively, our findings indicate that GS and GST expression levels and linoleic acid content are potentially good biomarkers for analyzing the effects of hypoxia.

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