Sebastián Zagmutt^{1, 2}, Elba Leiva³, Verónica Mujica⁴, Sergio Wehinger^4,

Introduction: Oxidative stress is one of the most important mechanisms in the emergence of type 2 diabetes. It would therefore be important to increase the antioxidant potential to prevent the deleterious effects of oxidative stress. Methods: MTT assay was performed to assess cell viability in the murine β TC-6 beta cell line. TBARs (thiobarbituric acid reactive substances) and GSH (glutathione) were measured and apoptosis were assessed by flow cytometry. Results: Exposure to 150 µM of H₂O₂ and 100 µM of tert-butyl hydroperoxide (t-BOOH) significantly reduced cell viability. When cells were simultaneously incubated with propolis extract (PE) and oxidants, cell viability relative to control was maintained. Exposure of cells to oxidants increased TBARs levels and reduced GSH concentration, a condition that was reversed when incubated with PE. A significant increase in apoptotic cells was seen when exposed to oxidants, however simultaneous incubation with PE reduced the number of apoptotic cells. Conclusion: PE has a protective effect against oxidative stress.

Polyphenolic, Propolis, Antioxidants, Oxidative stress, pancreatic beta cell