1Department of Food Science and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC
2Department of Food Science, Yuanpei University, Hsinchu, Taiwan, ROC
3Institute of Biological Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan, ROC
4Department of Health and Nutrition, Asia University, Taichung, Taiwan, ROC
Journal of Food and Nutrition Research.
2015,
Vol. 3 No. 8, 526-539
DOI: 10.12691/jfnr-3-8-8
Copyright © 2015 Science and Education PublishingCite this paper: Tai-Yuan Chen, Yu-Huai Chang, Hsi-Pin Lin, Shui-Tein Chen, Deng-Fwu Hwang. Proteomic Identification of Stonefish
Synanceja verrucosa Venom.
Journal of Food and Nutrition Research. 2015; 3(8):526-539. doi: 10.12691/jfnr-3-8-8.
Correspondence to: Deng-Fwu Hwang, Department of Food Science and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan, ROC. Email:
dfhwang@mail.ntou.edu.twAbstract
Three venom toxins, neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit, were identified in the stonefish Synanceja verrucosa by SDS-PAGE, Native-PAGE and two-dimensional electrophoresis (2-DE) coupled with Matrix Assisted Laser Desorption Ionization-Quadrupole-Time-of-Flight (MALDI-Q-TOF). The venom estimated by Native-PAGE were 471, 358, 260 and 166 kDa. The predominate protein bands of crude venom were 84 and 75 kDa by SDS-PAGE. The crude venom protein fell in the region with pI values of 7-9 and molecular weights of 75-90 kDa by 2-DE. Peptide mass fingerprints (PMF) and MS/MS ions originated from MALDI-Q-TOF were used to identify the protein. Our results showed that the complete components of neoverrucotoxin (neoVTX) α-subunit and β-subunit as well as verrucotoxin (VTX) β-subunit were identified from SDS-PAGE and 2-DE patterns. Native-PAGE did not yield protein identifications but revealed the presence of protein complexes.
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