Wen-Sing Liang^{1, 2}, Tristan C. Liu¹, Chun-Ju Chang¹, Chorng-Liang Pan^{1, 2,}

Oligosaccharides extracted from algae exhibit many bioactivities and are used as food additives and dietary supplements. In this study, β-1,3-xylan was extracted from the green algae Caulerpa lentillifera; this compound was hydrolyzed by β-1,3-xylanase XYLII to produce mixed < 3 kDa β-1,3-xylooligosaccharide (XOS_mix), which was mainly composed of β-1,3-xylose, β-1,3-xylobiose, and β-1,3-xylotriose. The antioxidant and anticoagulant activities of XOS_mix were then examined. Results revealed that the 2,2-diphenyl-1-pikryl-hydrazyl scavenging activity, reducing power, and total antioxidant status of 20 mg/mL XOS_mix was equivalent to those of 8.7, 115.1, and 157.3 μg/mL trolox, respectively; whereas the ferrous ion chelating activity of 20 mg/mL XOS_mix was equivalent to that of 64.3 μg/mL EDTA. Regarding the anticoagulant activity, XOS_mix delayed the activated partial thromboplastin time. These results suggest that XOS_mix exhibits potential for application in the food industry.

pseudomonas vesicularis MA103, β-1, 3-xylanase, β-1, 3-xylooligosaccharide, bioactivity