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Soukupova, J., Cvikrova, M., Albrechtova, J., Rock, R. N. and Eder, J. Histochemical and biochemical approaches to the study of phenolic compounds and peroxidases in Needles of Norway Spruce (Picea albies). New Phyto. 146, 403-414, 2000.

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Article

Histochemical Evidence of Dramatic Tissue Hardening in Post-harvest Trifoliate (D. dumetorum vars.) Yam Tubers

1Nutrition and Food science Department, University of Ghana, Box LG134, Legon, Ghana


American Journal of Food and Nutrition. 2015, Vol. 3 No. 3, 75-83
DOI: 10.12691/ajfn-3-3-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Bernard T. N. Otubuah, George S. Ayernor, Firibu K. Saalia. Histochemical Evidence of Dramatic Tissue Hardening in Post-harvest Trifoliate (D. dumetorum vars.) Yam Tubers. American Journal of Food and Nutrition. 2015; 3(3):75-83. doi: 10.12691/ajfn-3-3-3.

Correspondence to: Bernard  T. N. Otubuah, Nutrition and Food science Department, University of Ghana, Box LG134, Legon, Ghana. Email: botubuah@gmail.com

Abstract

Post-harvest hardening of Dioscorea dumetorum yam tubers are characterized by lignify cells. In this study, changes in phenolics in freshly harvested and appropriately stored (28±2°C; 72–85% RH and 5±1°C; 85-100% RH) trifoliate yam tubers using histochemical analysis were studied and related to tissue cohesion and hardening in white and yellow cultivars. Results showed an intense lignified cells (deep safranin red and toluidine blue stained cell wall areas) in most parenchyma tissues of stored white and yellow trifoliate yam tubers (28±2°C, 72-85%RH and 5±1°C, 85-100% RH). In addition, histochemical changes in parenchyma tissues were more moderately in phase with an intense tissue cohesion and hardening in white and yellow trifoliate yam tubers at 28±2°C; 72–85% RH and in yellow trifoliate yam tuber at 5±1°C; 85-100% RH. This finding has given insight into the role of lignified tissue in the hardening of post-harvest D. dumetorum yam tubers.

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