Rui Ma¹, Hantian Guo¹, Mengqing Guo¹, Shen Li¹, Liwen Tang¹, Yao Sun^1,

Many reports universal followed on common saponins of ginseng research field, few about functional identification of rare saponins. This study mainly researched new types of processed “one Steaming and one Sun-drying” Black Panax quinquefolius rare saponins (BPQRS) which extracted from Black Panax quinquefolius and its mechanism of inhibiting breast cancer cells. In our work, the extracts of Black Panax quinquefolius total saponins were analyzed used UPLC-Q/TOF-MS and 33 components were identified included 11 rare saponins. To further explored the inhibitory effect of BPQRS on disease pathways, network pharmacology and molecular docking simulation were carried out among 11 rare saponins, the rare saponins majoroside F6 and ginsenoside Rk1 expressed high correlation on inhibition pathway of breast cancer. Lots of reported discussed the function of ginsenoside Rk1, finally chose majoroside F6 of BPQRS to separated and purified by method of HSCCC. For identifying the function of majoroside F6, breast cancer cell MCF7 in vitro experiment confirmed that majoroside F6 exerted an significantly inhibiting cell proliferation by used MTT, flow cytometry and ELISA methods which upregulated caspase-3, Bax, inhibiting PI3K, AKT and Bcl-2 expression. This study laid a theoretical basis for developing and utilizing the medicinal value of BPQ.

One Steaming and one Sun-drying Black Panax quinquefolius, Inhibit breast cancer cell, Majoroside F₆, Network Pharmacology, UPLC-Q/TOF-MS