Qinshuai Ni¹, Zishan Liu¹, Haoyang Sun², Lin Zhu¹, Yan Zheng³, Yunliang Guo¹, Hongling Liu^4,, Tianwei Liu^1,

Objective To investigate the anti-liver-aging effect and mechanism of salidroside (Sal) against natural aging C57 mice by regulating the expression of telomerase reverse transcriptase (TERT). Methods A total of 50 C57 mice were randomly selected from 10 mice reared to 3 months old as the young control group, the other 40 mice were fed to 15 months of age as early aging mice, and were randomly subdivided into aging group, positive drug cycloastragenol (CAG) group, salidroside low-dose (Sal-L) and salidroside high-dose (Sal-H) group consisting of 10 mice each group respectively. The mice in young control group and aging group were given the same amount of normal saline 10 mL/kg intragastric administration, while the mice in positive drug group was given 20 mg/kg of CAG intragastric administration, and the mice in Sal-Land Sal-H groups were given 25 mg/kg and 100 mg/kg of salidroside respectively, once a day for 60 days. After the treatment, the aging degree of mice was scored. Colorimetric method was used to detect the activity of monoamine oxidase (MAO) in serum and the reactive oxygen species (ROS) content in serum. was detected by fluorescent probe method. The fibrosis of mouse liver tissue was observed by Masson staining and lipofuscin deposition was observed by aldehyde fuchsin staining. The protein expression of TERT was detected by Western Blot. Results Salidroside can reduce the aging score of aging mice, serum monoamine oxidase activity and the serum level of reactive oxygen species. The pathological staining indicated that salidroside could improve the aging degree of liver fibrosis and fat brown pigment deposition in mice.Western Blot results showed that the expression of TERT in liver tissue of aging mice increased after treatment with salidroside. Conclusion It is suggested that salidroside might delay the aging of mouse hepatocytes by up regulating the expression of TERT protein in the liver tissues of aging mice and inhibiting oxidative stress.

alidroside, senescence, hepatocyte, oxidative stress, telomerase reverse transcriptase