Shen Li¹, Liwen Tang¹, Mingye Ma¹, Yao Sun^1,, Mengqing Guo¹, Rui Ma¹

Corylus mandshurica (CM) was a shrub plant of the Betulaceae. It was not only edible but also could be used as medicine, widely used in traditional folk medicine. However, there were few studies on the medicinal value of CM Leaves(CML)the by-product of CM. In this article, a studied was carried out on hairy CML. The content of its compounds was determined and the extraction process was optimized. The optimum process conditions were obtained by single factor test and response surface optimization method was followed as ethanol concentration 70%, liquid-solid ratio 20ml/g, extraction temperature 60 °C and extraction time 2 h. In addition, the structures of 25 compounds in CML were identified by HPLC-ESI-MS / MS, including 14 flavonoids, 6 phenolic acids, 1 tannin, and 4 other compounds. The activities of anti-inflammatory, anti-tumor, and antibacterial of CML were studied. The results displayed that when the concentration of total flavonoids was 0.4 μg/ml and 4 μg/ml, they significantly inhibited the production of TNF-α, IL-6, and IL-1β in RAW264.7 induced by Lipopolysaccharide (LPS). The inhibitory effect of an ethanol extract from CML on the proliferation of Caco-2 cells was more potent than that on the expansion of SGC-7901 cells. The total flavonoid extraction of CML had different degrees of inhibition on Staphylococcus aureus and Escherichia coli, and the inhibition of Escherichia coli was greater than that of Staphylococcus aureus at the same concentration. This study laid a theoretical basis for developing and utilizing the medicinal value of CML.

flavonoid compounds, phenolic acid, anti-inflammatory, anti-tumor, antibacterial