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Song, F., Zuo, X., Zhao, Y., Li, Q., Tian, Z., and Yan, Y. “Betanin-enriched red beet extract attenuated platelet activation and aggregation by suppressing Akt and P38 Mitogen-activated protein kinases phosphorylation,” Journal of Functional Foods, 61, 2019.

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Article

Mitigation of LPS-Induced Inflammatory Responses Via Inhibition of NF-kB and MAPK Signaling Pathways by Betanin, a Coloring Agent

1Division of Gastroenterology and Hepatology, Chi Mei Medical Center, Tainan, 710402 Taiwan, R.O.C

2Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, 71710 Taiwan, R.O.C

3Research Institute of Biotechnology, Hungkuang University, 34 Chung-Chie Road, Shalu County, Taichung, 43302 Taiwan, R.O.C

4Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710 Taiwan, R.O.C


Journal of Food and Nutrition Research. 2024, Vol. 12 No. 1, 26-33
DOI: 10.12691/jfnr-12-1-3
Copyright © 2024 Science and Education Publishing

Cite this paper:
Ming-Jen Sheu, Shih-Ying Chen, Charng-Cherng Chyau, Ying-Chun Lin, Pin-Der Duh. Mitigation of LPS-Induced Inflammatory Responses Via Inhibition of NF-kB and MAPK Signaling Pathways by Betanin, a Coloring Agent. Journal of Food and Nutrition Research. 2024; 12(1):26-33. doi: 10.12691/jfnr-12-1-3.

Correspondence to: Pin-Der  Duh, Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710 Taiwan, R.O.C. Email: ipdduh@mail.cnu.edu.tw

Abstract

Betanin has been approved for use in food and pharmaceutical products as a natural red colorant. Recently, potential health benefits of betanin and betanin-rich foods have been discussed. This study aimed to investigate the attenuation of LPS-induced inflammatory responses through inhibition of NF-kB and MAPK signaling pathways by betanin. The results show that betanin at 1-50 µM exerted an anti-inflammatory effect on inhibiting LPS-induced intracellular nitric oxide (NO), prostaglandin E2 (PGE2), and interlukin 6 (IL-6). In addition, betanin decreased iNOS and COX-2 protein expression. Western blotting analysis indicated that betanin inhibited the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression and also inhibited the MAPK signaling pathway. Above all, anti-inflammatory effects of betanin are attributed to its inhibition of inflammatory gene expression through the blocking of the NF-kB and MAPK signaling pathways. Taken together, these findings suggest that betanin is a potential anti-inflammatory mediator for inflammation-related disease treatment.

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