Josyanne Araújo Neves¹, Francilene Vieira da Silva², Hélio Barros Fernandes², Ana Flávia Seraine Custódio Viana², Josynaria Araújo Neves², Celyane Alves Piauilino², Benedito Pereira de Sousa Neto², Joselma Sousa Lacerda Lopes², Aline Jeane Costa Sousa², Maísa Campelo de Sousa², Lucillia Rabelo de Oliveira Torres³, Julia López-Hernández⁴, Ana Rodriguez-Bernaldo de Quirós⁴, Raquel Sendón Garcia⁴, Rita de Cássia Meneses Oliveira^2,

The gastric ulcer is definited as erosion of the stomach's mucosal wall, which affects over 4.6 million people per year. The objective was to investigate the phenolic profile and the antioxidant activity of Melipona compressipes fasciculata pot-pollen collected at seasons rainy (PPMF1) and dry (PPMF2), as well as the gastroprotective and ulcer healing activities of PPMF2, as PPMF1 did not have a gastroprotective effect in screening protocols. The phenolic profile revealed compounds such as caffeic acid, quercetin, myricetin and naringenin. In models of gastric lesions by ethanol, ischemia/reperfusion (I/R) and acetic acid, PPMF2 reduced the lesion area (at doses 3 to 80 mg/kg, p.o.) and volume at doses (3 to 24 mg/kg, p.o.) with protection at percent 52 to 92%. PPMF2 too, significantly (p<0.05) elevated mucus and non-protein sulfhydryl groups (SH-NP) with an increase of 122% and 92% respectivelly compared to vehicle group, and decreased malonaldehyde (MDA) in rats (36% compared to vehicle group). As for cicatrizant activity in acetic acid-induced ulcer, PPMF2 promoted healing rates until 97%, caused significant microscopic gastric recovery, elevation of SH-NP (103%), attenuation of MDA (84%) levels and improvement of biochemical profile (p<0.05). This is a relevant and pioneering research associating stingless bee (M. compressipes fasciculata) in gastroprotective activity studies.

stingless bees, oxidative stress, phenolics, gastroprotection, pot-pollen