Article citationsMore >>

Ryu, D.S., Baek, G.O., Kim, E.Y., Kim, K.H. and Lee, D.S, “Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells,” BMB Rep, 43 (11). 750-5. Nov.2010.

has been cited by the following article:

Article

Anti-oxidant, Anti-inflammatory, and Anti-pancreatic Cancer Activities of Cynaroside and n-butanol Fraction of Orostachys japonicus

1Department of Smart Food and Drug, Graduate School, Inje University, Gimhae 50834, Republic of Korea

2Institue of Digital Anti-aging Healthcare, Graduate School, Inje University, Gimhae 50834, Republic of Korea;Department of Biomedical Laboratory Science, Inje University, 197 Inje-ro, Gimhae 50834, Gyeongsangnam-do, Republic of Korea


Journal of Food and Nutrition Research. 2023, Vol. 11 No. 6, 404-418
DOI: 10.12691/jfnr-11-6-2
Copyright © 2023 Science and Education Publishing

Cite this paper:
Sung Hyun Kim, Dong Seok Lee. Anti-oxidant, Anti-inflammatory, and Anti-pancreatic Cancer Activities of Cynaroside and n-butanol Fraction of Orostachys japonicus. Journal of Food and Nutrition Research. 2023; 11(6):404-418. doi: 10.12691/jfnr-11-6-2.

Correspondence to: Dong  Seok Lee, Department of Smart Food and Drug, Graduate School, Inje University, Gimhae 50834, Republic of Korea. Email: mbdslee@inje.ac.kr

Abstract

Cynaroside (luteolin-7-O-glucoside) is a flavone glycoside compound that has been reported to possess strong anti-oxidant activities. However, the integrative study for anti-inflammatory and anti-cancer activities of cynaroside alone or together with some flavonoids from medicinal herbs is rare. This study demonstrated anti-oxidant, anti-inflammatory, and anti-pancreatic cancer activities of cynaroside and n-butanol fraction derived from Orostachys japonicus (OJB). HPLC analysis indicated that OJB contained three different types of flavonoid glycosides; the major component among them was cynaroside. The anti-oxidant activities of cynaroside and OJB were validated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays and confirmed through analyzing the expression levels of heme oxygenase-1 (HO-1). The anti-inflammatory effects of them were certified by observing the decrease of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) in RAW 264.7 cells. Additionally, the anti-proliferative activities of them were confirmed by 3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium (MTS) assays in PANC-1 cells. Apoptotic bodies induced by them were clearly proven by DAPI staining. The cell cycle arrest at G1/S phase provoked by OJB was affirmed using flow cytometry. The effective activation of caspase -9, -8, -3 and inhibition of CDK2 and CDK4 by OJB were verified analyzing the protein levels measured using western blot. Such results indicated that both cynaroside and OJB effectively exerted anti-cancer effects on PANC-1 cells. In conclusion, the OJB containing cynaroside as a major flavonoid compound has the potential to be utilized as effectual anti-oxidant, anti-inflammatory, and anti-cancer agents in the future.

Keywords