Xiaofen Li^{1, 2}, Rongzhen Wang^{1, 2}, Kaiyuan Cui^{1, 2}, Hafeeze Noor^{1, 2}, Pengcheng Ding^{1, 2,}, Fida Noor¹

Low-temperature stress delays seed germination in maize. Different maize inbred lines display various low-temperature resistance, but the dynamic changes in seed germination under low-temperature stress in maize remain unknown, especially at the transcriptome level. Maize (Zea mays L.). 18 evaluation indexes of low temperature tolerance germination of 238 maize inbred lines were statistically analyzed, of which 12 evaluation indexes of low temperature tolerance had large coefficient of variation. The results of cluster analysis relative decrease of root length is similar to those of comprehensive evaluation cluster analysis of 12 low temperature tolerance indexes. The relative K + Q model was used to set the threshold - log10P = 4.61 to score the GWAS of evaluation indexes of low temperature tolerance. A total of 47 SNP loci significantly associated with low temperature tolerance were detected, of which the SNP loci significantly associated with chromosome 8 were the most, and the interpretation range of phenotypic contribution rate was 10.88% - 16.31%. It can be used as a candidate interval for subsequent screening and analysis. GWAS analysis showed that there were at least 2 evaluation indexes of low temperature tolerance, and a total of 6 SNPs were detected, of which 1 was on chromosome 5 and the other 5 were on chromosome 3. The consistent loci on chromosome 3 were located between PZE-103174386 (220,933,020) and PZE-103174950 (221,386,301).

seed germination, seedling growth, low-temperature stress, maize, peroxidase activity