1Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
2Department of Microbiology, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria
3Department of Biotechnology, School of Biological Sciences, Federal University of Technology, Owerri, Imo State, Nigeria
4Department of Statistics, College of Physical Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
American Journal of Food Science and Technology.
2021,
Vol. 9 No. 2, 30-37
DOI: 10.12691/ajfst-9-2-1
Copyright © 2021 Science and Education PublishingCite this paper: Onwuakor Chijioke E., Ogbulie Jude N., Braide Wesley, Ogbulie Tochukwu E., Nwokafor Chibuzo V., Uchendu C.E.. Optimization of Bacteriocin Production by
Lactobacillus fermentum Strain COE20 from Fermenting
Pentaclethra macrophylla Benth Using Response Surface Methodology.
American Journal of Food Science and Technology. 2021; 9(2):30-37. doi: 10.12691/ajfst-9-2-1.
Correspondence to: Onwuakor Chijioke E., Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria. Email:
ce.onwuako@mouau.edu.ngAbstract
This study evaluated the effect of varied culture conditions (Temperature, pH, and Sodium Chloride concentration) on bacteriocin production by Lactobacillus fermentum strain COE20 isolated from fermenting African oil bean seeds (Pentaclethra macrophylla Benth) using Response Surface Methodology (RSM). A Central Composite Design (CCD) was adopted with the interest of estimating the optimal conditions for its production using the response surface regression model, which estimated the linear, squared, and interactive relationship between the response variables. The Analysis of Variance (ANOVA) showed that the coefficient of determination in terms of predicted R2 was 0.8697 which was in close agreement with an adjusted R2 of 0.7393 and was accounted for by the predictors suggesting that the model was adequate. Optimal culture condition for bacteriocin production by L. fermentum strain COE20 was found at approximately 31°C, pH 5.9, 1.9% NaCl concentration at Y = 11.75mm. Y represents the response (zone of inhibition) against Staphylococcus aureus ATCC 19095 using the agar well diffusion assay method.
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