Jung-Ha Lee¹, Jae-Bong Lee², Jin-Tae Lee², Hae-Ryoun Park¹, Jin-Bom Kim^1,

Bromelain is obtained from Ananas comosus and is of significant clinical interest. Many studies have demonstrated bromelain's ability to treat immune-mediated conditions including reduction of platelet aggregation, angina pectoris and surgical trauma. ROS elimination were evaluated using a variety of anti-oxidation effect tests containing DPPH assay, ABTS+ radical scavenging assay, hydrogen peroxide scavenging assay. Western blotting analysis and RT-PCR were used to investigate an anti-inflammatory agent in LPS-induced RAW cells. Treatment with bromelain exhibited significant scavenging abilities on superoxide radicals compared with control. As results of analyzing iNOS and COX-2 band appearance according to bromelain treatment, it inhibited the band expression dose-dependently. Also bromelain inhibits the phosphorylated-MAP kinases signaling pathways. Furthermore, it reduced c-Fos, phosphorylation of c-Jun and c-Jun. According to the results, it is demonstrated that bromelain of Ananas comosus extract have significant anti-inflammatory ability as well as antioxidant ability.

bromelain, anti-oxidant effect, anti-inflammatory effect, public oral health