Article citationsMore >>

Flynn JM, Melov S. 2013. SOD2 in mitochondrial dysfunction and neurodegeneration. Free Radic Biol Med 62: 4-12.

has been cited by the following article:

Article

Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway

1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea

2Department of Bio and Chemical Engineering, Hongik University, Sejong, 30016, Korea


Journal of Food and Nutrition Research. 2018, Vol. 6 No. 9, 557-560
DOI: 10.12691/jfnr-6-9-3
Copyright © 2018 Science and Education Publishing

Cite this paper:
Jongsung Lee, See-Hyoung Park. Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway. Journal of Food and Nutrition Research. 2018; 6(9):557-560. doi: 10.12691/jfnr-6-9-3.

Correspondence to: See-Hyoung  Park, Department of Bio and Chemical Engineering, Hongik University, Sejong, 30016, Korea. Email: shpark74@hongik.ac.kr

Abstract

The anti-oxidant activity of daidzein was accessed by checking the expression level of superoxide dismutase 2 (SOD2) in HK-2 cell, a kidney proximal tubular cell line. SOD2 expression was increased in HK-2 cells treated by daidzein with a dose and time-dependent manner. Daidzein reduced the reactive oxygen species (ROS) level (about 60% compared to DMSO control with p = 0.005) in HK-2 cells. Daidzein caused FOXO3 to be translocated from cytosol into nucleus. Down-regulation of FOXO3 by transfection of siRNA against FOXO3 attenuated the expression of SOD2 and ROS level (p = 0.006) by daidzein. Taken together, this study has shown that daidzein displays anti-oxidant activity in HK-2 kidney proximal tubular cell line through up-regulation of FOXO3/SOD2 signaling pathway.

Keywords