This investigation attempted to study the antioxidant potential and phenolic acids profile in lettuce seeds (LS), the possibility of applying LS in pharmaceutical and food industry was also in focus. The antioxidant potential of LS extracts was determined. The total phenol content of LS extracts was 775.47 ± 118.58 mg GAE/100g whereas; the total flavonoid content was 170.83 ± 13.56 mg QE/100g. LS extracts showed a considerable scavenging activity against 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) as compared with standard ascorbic acid. The standard ascorbic acid showed an IC₅₀ of 12.32µg against DPPH, whereas the calculated IC₅₀ for LS extract required to scavenge DPPH was 1.74±0.11 mg. Phenolic acids in LS extracts were determined by using high performance liquid chromatography (HPLC).Gallic, chlorogenic, vanillic, caffeic, and coumaric acids were identified and quantified in LS extracts at a concentration of 20.30± 1.04, 1327.45± 45.67, 119.02± 4.88, 272.33± 12.77, and 912.15± 36.64 mg/100g respectively. Based on the obtained findings from the current research, lettuce seeds could have a potential for application in the pharmaceuticals and functional food industry.

Lettuce seeds (LS), Phenolic acids, High performance liquid chromatography (HPLC), Total phenolics, Total flavonoid , Scavenging activity