Wen-Chi Chen1, 2,
,
Huey-Yi Chen1, 2,
Wei-Yong Lin1, 2,
You-Rong Yang2,
Ming-Yen Tsai1, 3,
Yung-Hsiang Chen1, 4, 1Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan
2Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan
3Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan
4Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan;Department of Psychology, College of Medical and Health Science, Asia University, Taichung 41354, Taiwan
Journal of Food and Nutrition Research.
2018,
Vol. 6 No. 11, 706-709
DOI: 10.12691/jfnr-6-11-5
Copyright © 2018 Science and Education PublishingCite this paper: Wen-Chi Chen, Huey-Yi Chen, Wei-Yong Lin, You-Rong Yang, Ming-Yen Tsai, Yung-Hsiang Chen. Inhibitory Effect of Hydroxycitrate on Calcium Oxalate Crystal Formation in a
Drosophila Model.
Journal of Food and Nutrition Research. 2018; 6(11):706-709. doi: 10.12691/jfnr-6-11-5.
Correspondence to: Yung-Hsiang Chen, Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan. Email:
wgchen@mail.cmu.edu.tw, yhchen@mail.cmu.edu.twAbstract
In this study, we aimed to compare the effects of hydroxycitrate (HCA) and potassium citrate (PC) on the prevention and treatment of calcium oxalate (CaOx) stones in a fruit fly model. Drosophila melanogaster was used as an insect model of lithogenesis. The lithogenic agent used was 0.25% ethylene glycol. For determining the preventive effects, 2% PC and 2% HCA were added along with the lithogenic agent at the start of experiment. For determining the treatment effects, the lithogenic agent was added at the start of experiment to induce crystal formation, and 2% PC and 2% HCA were added from the third week. After 3 weeks, the Malpighian tubules of Drosophila were observed under polarized light microscopy, and the results were calculated. The preventive effect on the formation of CaOx in PC group was 9.38 ± 4.42% and in HCA group was 4.51 ± 3.85%. The treatment effect of PC was 56.90 ± 20.43% and that of HCA was 39.09 ± 15.36%. HCA has both preventive and treatment effects on the formation of CaOx crystals in the Malpighian tubules of Drosophila, and the effects were better than those of PC.
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