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Kuo, Y. C.; Weng, S. C.; Chou, C. J.; Chang, T. T.; Tsai, W. J. Activation and proliferation signals in primary human T lymphocytes inhibited by ergosterol peroxide isolated from Cordyceps cicadae. Br. J. Pharmacol. 2003; Volume 140, pp. 895-906.

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Fermented Cordyceps cicadae Mycelia Extracts Ameliorate Dry Eye Symptoms through Reduction of Cornea Epithelial Cell Apoptosis and Maintenance of Conjunctival Goblet Cells in a Mouse Dry Eye Model

1Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan

2Department of Nutrition, Chung Shan Medical University, Taichung City, Taiwan

3Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung City, Taiwan

4Institute of Food Science and Technology, National Taiwan University, Taiwan

5Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taiwan

6Department of Veterinary Medicine, National Chung Hsin University, Taiwan

7Department of Ophthalmology, Chung Shan Medical University Hospital, Taichung City, Taiwan


Journal of Food and Nutrition Research. 2017, Vol. 5 No. 5, 320-330
DOI: 10.12691/jfnr-5-5-6
Copyright © 2017 Science and Education Publishing

Cite this paper:
Tung-Yu Lin, Han-Hsin Chang, Yu-Jun Tang, Chin-Chu Chen, Li-Ya Lee, David Pei-Cheng Lin. Fermented Cordyceps cicadae Mycelia Extracts Ameliorate Dry Eye Symptoms through Reduction of Cornea Epithelial Cell Apoptosis and Maintenance of Conjunctival Goblet Cells in a Mouse Dry Eye Model. Journal of Food and Nutrition Research. 2017; 5(5):320-330. doi: 10.12691/jfnr-5-5-6.

Correspondence to: David  Pei-Cheng Lin, Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan. Email: pcl@csmu.edu.tw

Abstract

Cordyceps cicadae (Cc), a traditional Chinese medicine, has been shown to possess immunomodulatory and anti-inflammatory activities, and is regarded as having effects in vision improvement, but with no reported evidence. This study investigated the effects of Cordyceps cicadae fermented mycelia extracts (Cc extracts) in a benzalkonium chloride (BAC)-induced mouse dry eye model. Female ICR mice aged 6 weeks were randomly divided into four groups: blank, BAC-damaged without Cc extracts, BAC-damaged with 10 mg/kg bodyweight of Cc extracts, BAC-damaged with 100 mg/kg bodyweight of Cc extracts. The results showed that tear volume, tear film breakup time, and cornea surface indexes, including smoothness, opacity, topography, and the extent of lissamine green staining, were all improved with intake of Cc extracts intake, when compared to the status of the BAC-damaged group without Cc extracts. Immunohistochemical assays showed moderate change of Ki-67+ and Np63+ epithelial cell populations, while apoptotic epithelial cells, as detected by TUNEL assay, were decreased. PAS stain showed that the conjunctival goblet cell number and total cell area were decreased in the BAC-damaged group with Cc extracts at 10 mg/kg bodyweight. This study demonstrated that Cc extracts effectively ameliorate BAC-induced dry eye symptoms through enhancement of cornea resilience against BAC-induced damages and maintenance of conjunctival goblet cells.

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