eng Science and Education Publishing World Journal of Chemical Education 2375-1657 2023-11-07 11 4 134 140 10.12691/wjce-11-4-2 WJCE20231142 article Arijit Das arijitdas78chem@gmail.com 1 Digvijoya Sarmaa 1 Paresh Debnath 1 Bijaya Paul 2 Department of Chemistry, Bir Bikram Memorial College, Agartala, Tripura, India Department of Chemistry, School Education, Government of Tripura, Agartala-799001, India Chromatography is an essential physical technique that allows the constituent components of a mixture to be identified, separated, and purified in preparation for qualitative examination. Paper chromatography (PC) is a sort of planar chromatography, which refers to a stationary phase that is a solid, flat surface. In this illustration stationary phase is a particular kind of paper (Whatman quantitative filter paper grade 41). The fundamental idea behind paper chromatography is the differential passage of a mixture's constituent parts through filter paper or chromatography paper. A quick method for separating mixtures of metal ions, amino acids, carbohydrates, colors, and pharmaceuticals is paper chromatography (PC). For this qualitative analysis, only a very small sample is needed. Metal cation separation has seen increased by the use of the PC approach. Here, the four experiments show how PC may be used to separate metal ions [(Pb2+ & Cu2+), (Ni2+ & Co2+), (Cu2+ & Fe3+), (Cu2+ & Co2+)] based on their colored spots and the retardation factors or retention factors (Rf) values by using different eluting solutions. http://pubs.sciepub.com/wjce/11/4/2/wjce-11-4-2.pdf qualitative analysis green developer chromatographic jar spotting capillaries colored spots retention factor under graduate experiment