@article{wjac2013121,
author={AUTHOR = {Elbashir, Abdalla Ahmed and Alfadil, Amira Anwar Babikir},
title={Development and Validation of Spectrophotometric Method for Determination of Penicillamine (PA) in Pharmaceutical Formulation Using 4-Choro-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-CL)},
journal={World Journal of Analytical Chemistry},
volume={1},
number={2},
pages={18--22},
year={2013},
url={http://pubs.sciepub.com/wjac/1/2/1},
abstract={A sensitive and simple spectrophotometric method has been proposed for the determination of D-Penicillamine (PA) in pharmaceutical formulations. The proposed method is based on the reaction between the PA and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-CL) at alkaline medium (pH 10.5) to form deep brown-purple adduct, exhibiting maximum absorption (¦Ë<SUB>max</SUB>) at 468nm. Under optimized reaction condition, the method was linear in the concentration range 1-15¦Ìg mL<SUP>-1</SUP>. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.11¦Ìg mL<SUP>-1</SUP>, 0.38¦Ìg mL<SUP>-1</SUP>, respectively. The method was applied successfully to the determination of PA in pharmaceutical dosage form. A proposal of the reaction pathway has been postulated. The results were in a good agreement with those obtained with the official USP method. The method is useful for routine analysis of PA in quality control laboratories.},
doi={10.12691/wjac-1-2-1}
publisher={Science and Education Publishing}
}