eng Science and Education Publishing World Journal of Analytical Chemistry 2013-04-19 1 1 18 22 10.12691/wjac-1-2-1 WJAC2013121 article Abdalla Ahmed Elbashir hajaae@yahoo.com 1 Amira Anwar Babikir Alfadil 2 University of Khartoum, Faculty of Science, Chemistry Department, Khartoum, Sudan University of Sudan for Science & Technology, Faculty of Science, Department of chemical Laboratory, Khartoum, Sudan A sensitive and simple spectrophotometric method has been proposed for the determination of D-Penicillamine (PA) in pharmaceutical formulations. The proposed method is based on the reaction between the PA and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-CL) at alkaline medium (pH 10.5) to form deep brown-purple adduct, exhibiting maximum absorption (¦Ë_max) at 468nm. Under optimized reaction condition, the method was linear in the concentration range 1-15¦Ìg mL^-1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.11¦Ìg mL^-1, 0.38¦Ìg mL^-1, respectively. The method was applied successfully to the determination of PA in pharmaceutical dosage form. A proposal of the reaction pathway has been postulated. The results were in a good agreement with those obtained with the official USP method. The method is useful for routine analysis of PA in quality control laboratories. http://pubs.sciepub.com/wjac/1/2/1/wjac-1-2-1.pdf D-Penicillamine spectrophotometric pharmaceutical formulation method validation NBD-CL