eng Science and Education Publishing Research in Plant Sciences 2333-8539 2023-11-10 11 1 22 28 10.12691/plant-11-1-4 PLANT20231114 article Boukongo R. P. 1 Elion Itou RDG romaricelion@gmail.com 1 2 3 Mambeke H. M. 3 Etou Ossibi AW 3 3 Morabandza C. J. 4 Abena AA 4 Laboratory of Pharmacodynamics and Experimental Pathophysiology (L2PE), Marien University Ngouabi, BP69, Brazzaville, Congo Department of Natural Sciences, Ecole Normale Sup¨¦rieure, Marien University, Ngouabi Congo, B.P 69, Brazzaville, Congo Intestinal spasms are violent contractions that occur in the intestine. Aside from treatment with conventional medications, certain medicinal plants are widely used alone or in combination in traditional congolese medicine to treat spasms. This study aims to evaluate the antispasmodic effects of an aqueous extract of the recipe made from the leaves of O. gratissimum and the bark of T. superba. The spasms were induced 1 hour after administration of the aqueous extract by intraperitoneal injection of 1% acetic acid; the duration of the experiment varied from 30, 60 and 90 minutes. Then, the animals were sacrificed after anesthesia with ethyl ether, the peritoneum was rinsed with 0.9% Nacl, the peritoneal fluid obtained in order to measure prostaglandins (PGE2¦Á and PGF2¦Á), as well as oxidative stress parameters. The aqueous extract at the doses (125,250 and 500 mg/kg) used has an antispasmodic effect which resulted in a significant reduction (p<0.001) in the number of spasms. The antispasmodic effects were confirmed by a significant decrease (p<0.001) in prostaglandins PGE2¦Á and PGF2 for their involvement in the pain induction mechanism of acetic acid. Furthermore, a significant reduction (p<0.001) in the parameters of oxidative stress induced by the intraperitoneal injection of acetic acid was observed. The results showed that the aqueous extract of the recipe has significant antispasmodic activity and could justify the traditional use of the recipe in abdominal pain. https://pubs.sciepub.com/plant/11/1/4/plant-11-1-4.pdf spasms antispasmodic O gratissimum T superba recipe