@article{jpbpc20241212,
author={Kasaai, Mohammad Reza},
title={How Do We Set Up or Choose An Optimal Procedure to Determine the Molecular Weight of Polymers with A Good Accuracy Using Size Exclusion Chromatography or Viscometry? A Mini-Review},
journal={Journal of Polymer and Biopolymer Physics Chemistry},
volume={12},
number={1},
pages={8--14},
year={2024},
url={https://pubs.sciepub.com/jpbpc/12/1/2},
issn={2373-3411},
abstract={Several procedures have been already developed to determine the average-molecular weight (M) for polymers. However, determination of M with an optimal procedure, given a good accuracy is a major issue for research groups. This mini-review described three following procedures for determination of M with a good accuracy: (a) viscometry using intrinsic viscosity ([&#951;]) data and Mark¨CHouwink¨CSakurada (MHS) equation; (b) size exclusion chromatography (SEC) with a single concentration detector; and (c) SEC equipped with two detectors, concentration and viscometry detectors. The following conclusions were made: (1) in viscometry, the accuracy of M depends on the accuracy for the MHS equation constants, <i>K a</i>nd <i>a</i>. The values <i>K </i>and <i>a</i>, depend on the nature of polymer, quality of the solvent, polydispersity of the polymer samples, molecular weight range, and temperature. of polymer solution; (2) in SEC with a single detector, the value of M was determined, based on the calibration curve (log M against retention time). The polymer standard should have the same conformation in solution with the polymer under investigation, otherwise an unsatisfactory value for the M will be obtained; and (3) SEC equipped with the two detectors, the value of M for the investigated polymer was determined with a good accuracy. The polymer could have any conformation in solution either similar or different from the polymer standard.},
doi={10.12691/jpbpc-12-1-2}
publisher={Science and Education Publishing}
}