eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2019-03-02 7 3 187 190 10.12691/jfnr-7-3-2 JFNR2019732 article Hyun Woo Kang khw7200@ysu.ac.kr 1 Sang Jae Kwak 1 Department of Korean Food & Culinary Arts, Youngsan University, Busan, 48015, Korea The antioxidative activity of extracts from Juniperus rigida Sieb. Fruit (JRSF) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The free radical scavenging activity of the extract was dose dependant manner, and the IC50 value on DPPH, hydroxyl and alkyl radical at 16.84, 82.35 and 52.27 μg/mL respectively. In addition, evaluated the protection effect on H2O2-induced oxidative damage in PC-12 cells via propidium iodide (PI) staining using a flow cytometer. JRSF extract decreased cell death in PC-12 cells due to H2O2-induced oxidative damage in a dose-dependent manner. These results suggest that JRSF extract exhibit antioxidative activity against oxidative stress on PC-12 cells. http://pubs.sciepub.com/jfnr/7/3/2/jfnr-7-3-2.pdf Juniperus rigida Sieb fruit antioxidant free radical PC-12 cells flow cytometer