eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2019-02-03 7 2 96 104 10.12691/jfnr-7-2-1 JFNR2019721 article Seba Harphoush 1 2 3 Guoqing Wu 3 3 Margaret Zaitoun 3 3 3 Yonghui Shi 3 3 Guowei Le lgw@jiangnan.edu.cn 3 3 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China Methionine restriction has been proven to reverse age and obesity related metabolic diseases. Basically, MR regulates energy homeostasis in the whole body contributing to enhanced insulin sensitivity and improved redox status. There is no studies until now that comprehensively focus on the impact of MR on reproduction performance. The aim of this work was to investigate the reproductive system response of female obese model toward MR and clarify the possible mechanism. Females C57BL/6 were subjected to control diet (CON, 0.86% methionine + 4% fat), High Fat Diet (HFD, 0.86% methionine + 20% fat), High fat methionine Restricted diet (MR, 0.17% methionine + 20% fat). HFD showed impaired reproduction performance characteristic by impaired conception ability and lactation difficulties. MR improved oxidative stress, metabolic parameters, mammary and ovarian morphology. MR enhanced ovulation, lactation, and especially regulated metabolism efficiency via AMPK/SIRT1/PGC1. This study is the first to suggest that MR can ameliorate reproductive disorders induced by obesity. http://pubs.sciepub.com/jfnr/7/2/1/jfnr-7-2-1.pdf methionine restriction high fat diet obesity reproduction metabolic dysfunction