@article{jfnr2015326,
author={{Tsai, Kao-Sung and Chen, Yung-Hsiang and Shen, Jui-Lung and Man, Kee-Ming and Wu, Sun-Yuan and Chen, Huey-Yi and Chang, Chiao-Hui and Lee, Yuan-Ju and Hsu, Tzu-Fang and Tsai, Fuu-Jen and Lin, Wei-Yong and Chen, Wen-Chi},
title={Does Chronic Cola Consumption Increase Urinary Stone Risk? Evidence from the <i>Drosophila</i> Model of Urolithiasis},
journal={Journal of Food and Nutrition Research},
volume={3},
number={2},
pages={109--113},
year={2015},
url={http://pubs.sciepub.com/jfnr/3/2/6},
issn={2333-1240},
abstract={There are rising public health concerns about the links between consumption of sugar-sweetened beverages and weight gain, obesity, and other metabolic problems. Sodium citrate (Na-citrate) is used as an additive in colas and various commercial drinks worldwide. Although potassium citrate (K-citrate) has been prescribed and well accepted by urologists for treating urinary stone disease (urolithiasis), the clinical role of Na-citrate has not been well established. We investigated the effects of Na-citrate and cola on the treatment of urolithiasis with an emerging translational model ¨C <i>Drosophila melanogaster</i>. <i>Drosophila</i> medium containing 0.5% ethylene glycol (EG) was used as a lithogenic agent for calcium oxalate (CaOx) crystal formation in <i>Drosophila</i> Malpighian tubules. Cola (25%) and Na-citrate (2% and 4%) were added to the fly medium for urolithiasis treatment. Medium containing K-citrate (2% and 4%) was used as a positive control. After 3 weeks of treatment, the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination; fly lifespan was also monitored in different groups.<b> </b>Cola failed to reduce CaOx crystal formation, whereas Na-citrate and the positive control K-citrate significantly reduced EG-induced CaOx crystal formation in <i>Drosophila</i>. Administration of either Na-citrate or cola did not inhibit <i>Drosophila</i> lifespan. Consumption of cola exerts no detectable change in the lithogenic agent associated with CaOx stone formation in the <i>Drosophila</i> model. By contrast, Na-citrate had an inhibitory effect on EG-induced CaOx crystal formation, albeit a lower inhibitory rate upon comparison with K-citrate.},
doi={10.12691/jfnr-3-2-6}
publisher={Science and Education Publishing}
}