eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2025-02-04 13 1 34 42 10.12691/jfnr-13-1-4 JFNR20251314 article Zhongshan Xiao 1 Zhenglin Chen 2 Siru Chen 2 Xiaoman Yang 3 Tianlin Wang wangtianlin@henau.edu.cn 3 Department of Pharmacy, Puyang Medical College, Puyang, Henan, China College of Food Science and Technology, Henan Agricultural University, Zhengzhou, China Puyang People's Hospital, Puyang, Henan, China The transformation of white to beige adipocytes is a promising method for the treatment of obesity. The aim of this study was to investigate the effect of stachyose on the regulation of adipocytes browning. The 3T3-L1 preadipocytes and C3H10T1/2 multipotent stem cells were differentiated into mature adipocytes with stachyose (0.5, 2.0 mg/mL). Results showed that the treatment of stachyose significantly decreased intracellular lipid accumulation and increased mitochondrial contents in 3T3-L1 adipocytes and C3H10T1/2 cells. Stachyose, which led to an increase of the relative numbers of UCP1-positive cells, has not only promoted the expression of browning-specific genes and proteins but also induced the phosphorylation of AMPK. Be-sides, the addition of AMPK inhibitor (compound C) has reduced the increase in the protein expression of thermogenesis-related markers induced by stachyose, suggesting that AMPK could affect the stachyose-mediated adipocyte browning process. These results indicate that stachyose has a probable effect on the regulation of adipogenesis and prevention of obesity. https://pubs.sciepub.com/jfnr/13/1/4/jfnr-13-1-4.pdf Stachyose Adipocytes Browning UCP1 AMPK Obesity