eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2025-01-15 13 1 9 17 10.12691/jfnr-13-1-2 JFNR20251312 article Sudhanva M S 1 Akhina Tom 2 Firoz H M 2 Zayeem Firoz Hussain 3 Sadashiva CT research@starhiherbs.com, Shobithrangappa@gmail.com 3 Shobith Rangappa research@starhiherbs.com, Shobithrangappa@gmail.com 3 Adichunchanagiri Institute for Molecular Medicine, Adichunchanagiri University, B G Nagara- 571 448, Mandya Department of Research and Development, Star Hi Herbs Pvt Ltd, Jigani, Bangalore-560105, Karnataka, India Department of Biotechnology, The Oxford College of Engineering, Bangalore, Karnataka, India Obesity is gradually becoming a widespread health problem, and treatment using natural compounds has seen an increasing trend. The aim of this study investigated the effects and underlying mechanisms of Cissus quadrangularis (Cissuslean®) on adipogenesis and high-fat diet (HFD)-induced obesity. The inhibitory effects of Cissuslean® on adipocyte differentiation were evaluated in vitro using 3T3-L1 preadipocyte cells, focusing on AMPK signaling and lipid metabolism. Subsequently, an in vivo trial with male C57BL/6J mice fed HFD was conducted to examine the effects of Cissuslean® at 25, 50, and 100 mg/kg on adipose tissue characteristics, body weight, fat mass, and obesity-related gene expression. Cissuslean® significantly inhibited preadipocyte differentiation by reducing intracellular lipid accumulation and suppressing the expression of key adipogenic regulators. It also enhanced AMPK phosphorylation during early adipocyte differentiation. In HFD-induced obese mice, Cissuslean® administration resulted in marked reductions in body weight gain, adipose tissue mass, and hepatic lipid accumulation. Furthermore, decreased serum ALT and AST levels indicated attenuation of HFD-induced hepatic injury. According to these results, Cissuslean® reduces body fat mass by inhibiting lipase activity and regulating genes and proteins involved in adipogenesis and lipogenesis in epididymal adipose tissue. Our results suggest that Cissuslean® may be used as an anti-obesity agent. https://pubs.sciepub.com/jfnr/13/1/2/jfnr-13-1-2.pdf Anti-obesity Cissus quadrangularis Cissuslean? DEXA high-fat diet