@article{jfnr20241223,
author={{Li, Shen and Tang, Liwen and Ma, Mingye and Sun, Yao and Guo, Mengqing and Ma, Rui},
title={Study on Extraction, Identification, and Biological Activity of Compounds in Leaves of <i>Corylus mandshurica</i>},
journal={Journal of Food and Nutrition Research},
volume={12},
number={2},
pages={85--97},
year={2024},
url={https://pubs.sciepub.com/jfnr/12/2/3},
issn={2333-1240},
abstract={<i>Corylus mandshurica</i> (CM) was a shrub plant of the <i>Betulaceae</i>. It was not only edible but also could be used as medicine, widely used in traditional folk medicine. However, there were few studies on the medicinal value of <i>CM Leaves</i>(CML)the by-product of <i>CM</i>. In this article, a studied was carried out on hairy <i>CML</i>. The content of its compounds was determined and the extraction process was optimized. The optimum process conditions were obtained by single factor test and response surface optimization method was followed as ethanol concentration 70%, liquid-solid ratio 20ml/g, extraction temperature 60 ¡ãC and extraction time 2 h. In addition, the structures of 25 compounds in <i>CML</i> were identified by HPLC-ESI-MS / MS, including 14 flavonoids, 6 phenolic acids, 1 tannin, and 4 other compounds. The activities of anti-inflammatory, anti-tumor, and antibacterial of <i>CML</i> were studied. The results displayed that when the concentration of total flavonoids was 0.4 ¦Ìg/ml and 4 ¦Ìg/ml, they significantly inhibited the production of TNF-¦Á, IL-6, and IL-1¦Â in RAW264.7 induced by Lipopolysaccharide (LPS). The inhibitory effect of an ethanol extract from CML on the proliferation of Caco-2 cells was more potent than that on the expansion of SGC-7901 cells. The total flavonoid extraction of <i>CML</i> had different degrees of inhibition on <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, and the inhibition of <i>Escherichia coli</i> was greater than that of <i>Staphylococcus aureus</i> at the same concentration. This study laid a theoretical basis for developing and utilizing the medicinal value of CML.},
doi={10.12691/jfnr-12-2-3}
publisher={Science and Education Publishing}
}