eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2024-10-14 12 10 399 408 10.12691/jfnr-12-10-1 JFNR202412101 article Thi Hanh Nguyen hanh.nguyenthi@hust.edu.vn 1 Ngoc Cham Nguyen 1 Thi Trang Nguyen 1 Van Hung Nguyen 1 Department of Food Engineering, School of Chemistry and Life Sciences, Hanoi University of Science and Technology, Hanoi, Vietnam Soursop (Annona muricata L.) of Annonaceae is a great kind of nutritional and biological potential tropical plant. Fermented fruit products are currently a popular trend in consumption due to their various advantages. Nowadays, in addition to commonly used fermentation methods including alcoholic fermentation, acetic fermentation and lactic fermentation, multi-strain fermentation (or symbiosis) is increasingly applied in food production, especially the fruit juice sector. Through the process of altering the fermented conditions of this research, the appropriate process applied was a combination of 48-hour lactic fermentation with the addition of 107 CFU/mL L. plantarum bacterial strain at 30°C; followed by 24-hour combined alcoholic and lactic fermentation with the addition of 106 CFU/mL S. bayanus yeast strain at 15°C. The suitable conditions for fermentation of soursop fruit juice were established as the dilution ratio of soursop juice (SJ) and water (W) of 1:2; the total soluble solids (TSS) of 16°Brix; pH 5.0. Under these suitable fermentation parameters, the final product achieved total titratable acidity (TTA) of 0.488 ± 0.026%, total sugar content of 14.881 ± 0.189 g/100mL, TSS of 15.37 ± 0.17 oBrix, pH index of 3.82 ± 0.04, ethanol content of 0.42 ± 0.01 %v/v, vitamin C concentration of 29.26 ± 0.79 mg/100mL and total phenolic content of 95.35 ± 0.64 mg GAE /100mL. After assessment, the fermented soursop juice met the required microbiological quality standards. The sensory quality of the cocultured fermentation soursop juice was rated moderated like (7.41 ± 0.46 on 9-point hedonic scale). https://pubs.sciepub.com/jfnr/12/10/1/jfnr-12-10-1.pdf Soursop multi-strain fermentation fruit juice Lactobacillus plantarum Saccharomyces bayanus