eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2023-09-18 11 9 573 578 10.12691/jfnr-11-9-3 JFNR20231193 article Qiao Peng 1 Guo Jingli 240060329@qq.com 1 Wang Yingtao 1 Wang Jianen 1 Fida Noor 2 Waseem Bashir 3 Sana Ullah 4 Henan Xinlianxin Chemicals Group Co., Ltd. Xinxiang, 453700, China College of Veterinary Medicine, Shanxi Agriculture University, Taigu, 030801, Shanxi, China Lasbela University of Agriculture, Water & Marine Sciences (LUAWMS) Balochistan, Pakistan University College of Dera Murad Jamali Naseerabad (LUAWMS), Balochistan,Pakistan This study aimed to evaluate the biochemical impact of nitrogen (N) fertilization on different wheat cultivars. The experiment included two varieties (YH-618 and YH-20410) and three nitrogen levels, (N0, N210, and N280). Our results indicated that wheat variety YH-20410 had the higher nitrogen uptake and efficiency, grain protein content, and yield at higher planting densities and will benefit farmers by forming stronger overall crops. For variety YH-20410, the soil water storage at the wintering stage was significantly highest at N280 variety YH-20410, wheat higher dry matter accumulation of each organ at maturity stage, and the dry matter accumulation of leaf, stem + leaf sheath. Compared to N0 and N180, N210 significantly increased the N harvest index by 5.0% to 19.4% and N use efficiency by 2.9% to 9.1%, but there was no significant difference in N uptake efficiency. Variety YH-20410 was beneficial to improve the harvest index and N productivity of wheat. Variety YH-20410, has also the greater number of spike number, grains per spike and 1000-grain weight, leading to high grain yield. Among nitrogen treatments, N280 significantly increased the grain number per spike, 1000-grain weight and yield. Significantly increased grain gluten content, grain protein content and protein yield under of YH-20410 variety. In conclusion, reduced N fertilizer 280 and variety YH-20410 was beneficial to the improved the leaf area index, plant height, soil water storage, dry matter accumulation, and grain yield. https://pubs.sciepub.com/jfnr/11/9/3/jfnr-11-9-3.pdf wheat nitrogen dense interaction particle size distribution viscosity parameter