@article{jfnr202311116,
author={{Zi-shan, Liu and Yan-xue, Zhang and Xiao-lin, Wu and Ning, Gu and Xin-ying, Xu and Zhu-qin, Yu},
title={The Neuroprotective Effect and Possible Mechanism of Picroside II Against Oxygen Glucose Deprivation/Reoxygenation Cell Model},
journal={Journal of Food and Nutrition Research},
volume={11},
number={11},
pages={700--706},
year={2023},
url={https://pubs.sciepub.com/jfnr/11/11/6},
issn={2333-1240},
abstract={Objective: This study aims to delineate whether autophagy plays an critical role in the neuroprotective effects of picroside II in vitro and further explore potential molecular mechanism. Methods: The oxygen glucose deprivation/reoxygenation (OGD/R) cell model were established in SH-SY5Y cells. The cell viability, cells morphologic change and apoptosis were observed by cell counting kit-8 (CCK-8), inverted microscope and flow cytometry respectively. Autophagy-related proteins Beclin 1, microtubule associated protein 1 light chain 3 (LC3), and p62 levels of brain tissues and cells were detected by Western Blot; furthermore, LC3 expression and autophagy flux of cells were further detected by immunofluorescence labeling and GFP-mRFP-LC3 adenovirus transfection. Reactive oxygen species (ROS) levels and phospho-AMPK, phospho-mTOR, phospho-ULK 1 levels were were detected using ROS assay Kit, Western blot and immunocytochemistry, respectively. Results: Picroside II down-regulated of autophagy-related Beclin-1 and LC3 levels, and up-regulated of p62 protein; meanwhile ameliorated the abnormal morphological structures of nerve cells and apoptosis; and increased cell viability. Furthermore, accompanied by decreasing autophagy flux, picroside II prevented the generation of ROS, down-regulated of AMPK and the up-regulated of mTOR and Unc-51-like kinase 1 (ULK1) levels. Conclusion: Picroside II exerts neuroprotective effect against OGD/R injury by inhibiting ROS-mediated AMPK-mTOR-ULK1 autophagy signaling pathway},
doi={10.12691/jfnr-11-11-6}
publisher={Science and Education Publishing}
}