@article{jfnr20221013,
author={{Tahir, Usaal and Zameer, Mariam and Zahra, Nureen and Mazhar, Maham and Rafique, Ayesha and Alyas, Sobia and Shabbir, Aroosh and Mehreen, Arifa and Shafiq, Muhammad Imtiaz and Mazhar, Modasrah and Akram, Qaiser},
title={Isolation and Characterization of Shiga-toxigenic <i>Escherichia coli</i> Isolated from Various Food Samples},
journal={Journal of Food and Nutrition Research},
volume={10},
number={1},
pages={19--25},
year={2022},
url={http://pubs.sciepub.com/jfnr/10/1/3},
issn={2333-1240},
abstract={Shiga toxigenic <i>Escherichia coli </i>(STEC) is a food-borne pathogen associated with community health concern. Contamination of food by STEC and toxin causes infectious diseases. <i>Escherichia</i><i> coli</i> isolated from food samples such as yogurt, apples, tomatoes and milk by applying violet-red bile glucose agar, Triphenyl tetrazolium chloride agar (TTC) and eosin-methylene blue agar (EMB) test after pre-enrichment of food samples in buffered peptone water. <i>Escherichia coli</i> isolates were screened for the existence of shiga toxin genes <i>Stx1</i> and <i>Stx2</i> through Polymerase Chain Reaction (PCR). Apples (17%), tomatoes (26%) and milk (1%) of the samples examined showed positive results for <i>Stx</i> genes. Yoghurt samples examined in this study had no <i>Stx1</i> or <i>Stx2</i> gene. The presence of Shiga toxin that is protein in nature was confirmed by SDS-PAGE in milk sample. The present study has shown that foods from densely populated areas i.e. Harbanspura, Lahore could be sources of STEC.},
doi={10.12691/jfnr-10-1-3}
publisher={Science and Education Publishing}
}