Science and Education Publishing Journal of Applied & Environmental Microbiology 2373-6712 3 5 2016 1 5 Production and Estimation of Keratinase by Immobilized and Free Bacillus licheniformis (St. 24) 119 122 EN Farag M. Saieb Salama A. Boaker Hasham M. El-komy Ahmed Issa Biology Department, Faculty of Science, Taif University, Taif KSA JAEM2015352 10.12691/jaem-3-5-2 2015 10 5 2015 11 24 2016 1 3 Alginate immobilized Bacillus licheniformis (St. 24) were isolated from chicken feather wastes recorded higher keratinase production than free bacterial suspension. 2-3% alginate concentration, medium pellet size, and in addition of keratin powder as adjuvant were optimum for keratinase production by the immobilized bacteria. Encapsulated B. licheniformis st. 24 was successfully produced keratinase for three repeated batch fermentation cycles each 24 hour incubation period. Immobilization of the whole cells proved to be useful for continuous production of keratinase and feather degradation.