eng Science and Education Publishing Journal of Applied & Environmental Microbiology 2373-6712 2025-11-10 13 1 33 40 10.12691/jaem-13-1-5 JAEM20251315 article Toualy Serge Thibaut OUINA ouitoualy@yahoo.fr 1 Rosin Don Rodrigue VOKO Bi 1 Kouam¨¦ Claude YA 1 Pascaline Emira Wilfried KOUAM¨¦ 1 Marina KOUSSEMON-CAMARA 2 Ibrahim KONATE 2 Laboratory of Agrovalorisation, Department of Biochemistry and Microbiology, University Jean LOROUGNON GUEDE, Daloa, C?te d¡¯Ivoire Laboratory of Biotechnology and Food Microbiology, Department of Food Sciences and Technology, University Nangui Abrogoua, Abidjan, C?te d¡¯Ivoire Salinisation due to intensive agriculture and geological alteration reduces soil microbial activity, creating unfavorable conditions for plant growth. This study aimed at screening degraded banana plantation soils from Digbapia and Zokoguhé (Daloa, Côte d¡¯Ivoire) for fluorescent Pseudomonas spp. able to tolerate saline stress. Isolates were recovered, assayed for extracellular enzymatic activities, and tested for growth across a NaCl and KNO3 gradient up to 10%. Soil samples were collected from six degraded banana plantation sites. Fluorescent Pseudomonas spp. were isolated using culture techniques on media King¡¯s A and B. Isolates were subsequently assayed for amylase, cellulase, protease and lipase activities and evaluated by monitoring growth in media supplemented with NaCl and KNO3 at concentrations ranging from 0 to 10%. Eight Pseudomonas species coded C226, C211, C127, C213, C212, C112R, C2211 and C129 were isolated from these soils. Among these isolates, C112R and C226 showed amylolytic activity, while C212, C213 and C127 showed cellulolytic activity. For high concentrations (8 to 10%), in KNO3 between 791.27 and 989 mM then in NaCl between 1368.9 and 1711.1 mM, the isolates able to grow were C129, C213, C127, C2211 and C112R. These species are the best candidates for resistance to salt stress. They could serve as plant biostimulants under saline conditions, contributing to soil restoration. https://pubs.sciepub.com/jaem/13/1/5/jaem-13-1-5.pdf salinity Pseudomonas enzymes degraded soil agriculture