eng Science and Education Publishing American Journal of Pharmacological Sciences 2327-672X 2024-05-08 12 2 17 20 10.12691/ajps-12-2-1 AJPS20241221 article Kingsley Ofolikwei Quaye 1 Dickson Aboagye dicksonaboagye184@gmail.com 2 Herman Caesar Sung-Bawiera Azaanang 2 Joseph Nii Amon Dodoo 2 Eugenia Serwaa Nyampong 2 Josephine Oppong Frimpomaa 2 Caleb Ofori Bandoh 2 Department of Science Laboratory Technology, Accra Technical University, Accra, Ghana Department of Pharmaceutical Science, School of Pharmacy, Central University, Accra, Ghana There is an increasing trend in the study of the anti-inflammatory properties and phytochemical content of herbal medicines worldwide. Literature is however lacking on the effects of heat treatment during the processing of medicinal plants of their bioactivities and phytochemical content. This study seeks to fill this gap by investigating how temperature affects the anti-inflammatory properties of Moringa oleifera leaves. The plants were harvested and ground into a powder, after which aqueous and ethanol extracts were conducted. The extract was freeze-dried for further examination. The anti-inflammatory assay activity shows that the IC50 values of moringa leaves at room temperature, 60°C, and 90°C were 0.1313±0.0269, 0.0939±0.0901l, and 0.0757±0.0162 respectively. Diclofenac sodium, a standard anti-inflammatory drug showed an IC50 value of 0.1022±0.0204. The study also showed that temperature influenced the phytochemical components in the moringa leaf extract. Phytochemical components such as reducing sugars, saponins, phenols, flavonoids, triterpenes, and phytosterols were all present. https://pubs.sciepub.com/ajps/12/2/1/ajps-12-2-1.pdf herbal medicine anti-inflammatory phytochemicals temperature