@article{ajphr20251352,
author={{Elhassaneen, Yousif A. and Darwish, Amira H. and Mosa, Alaa A.},
title={Protective Effects of Lady¡¯s Mantle (<i>Alchemilla vulgaris</i> L.) against Hepatotoxicity in a Rat Model: Insights into Active Compounds and Underlying Mechanisms},
journal={American Journal of Public Health Research},
volume={13},
number={5},
pages={208--223},
year={2025},
url={https://pubs.sciepub.com/ajphr/13/5/2},
issn={2327-6703},
abstract={<i>Alchemilla vulgaris</i> (Lady¡¯s mantle) has shown various therapeutic properties, yet its liver-protective potential remains underexplored. This study aimed to evaluate its hepatoprotective effects in a rat model of CCl?-induced liver toxicity and to characterize its bioactive compounds. Thirty rats were divided into five groups: a negative control, a CCl?-induced positive control, and three treatment groups receiving ethanol extract of Lady¡¯s mantle (AVE) at doses of 150, 300, and 450 mg/kg body weight/day. Proximate analysis of the aerial parts revealed 39.86% carbohydrates, 30.92% crude fiber, 15.94% protein, 10.83% ash, and 2.45% fat. The ethanol extract was rich in bioactive compounds, including phenolics (621.67 ¦Ìg GAE/g), terpenoids (411.52 ¦Ìg/g), anthocyanins (214.66 ¦Ìg/g), flavonoids (181.45 ¦Ìg/g), and triterpenoids (129.34 ¦Ìg/g). AVE exhibited strong antioxidant activity (87.34%), with an IC50 of 14.42 ¦Ìg/mL. In the biological study, AVE treatment dose-dependently improved body weight gain, food intake, and feed efficiency ratio in hepatotoxic rats. Liver function markers also improved, with glycogen content increasing by 128.64%, G6PD activity by 95.78%, and G6Pase activity decreasing by 53.25% at the highest dose. Serum lipid profiles were corrected, with HDL-c increasing by up to 65.06%, LDL-c decreasing by 32.52%, and total cholesterol dropping by up to 9.42%. Antioxidant defenses were restored as reduced glutathione increased by 89.49%, while oxidative stress markers such as ROS and MDA decreased by up to 53.61% and 26.01%, respectively. Histopathological analysis confirmed reduced liver damage and inflammation in treated groups. In conclusion, <i>Alchemilla vulgaris</i> ethanol extract demonstrates potent antioxidant and hepatoprotective properties. Further investigation may support its use as a natural therapeutic agent for liver disorders.},
doi={10.12691/ajphr-13-5-2}
publisher={Science and Education Publishing}
}