eng Science and Education Publishing American Journal of Medical Sciences and Medicine 2327-6657 2025-12-22 13 4 81 86 10.12691/ajmsm-13-4-6 AJMSM20251346 article Kiyindou-Soungadia L.F.C 1 Wossolo Lingomo B.S 1 2 3 Lebonguy A.A 4 Etou Ossibi A.W etouarnaud@yahoo.fr 4 4 Laboratory of Pharmacodynamics and Experimental Physiology, Faculty of Science and Technology, Marien Ngouabi University, BP 69, Brazzaville, Congo Microbiology Unit, National Institute for Research in Exact and Natural Sciences, Brazzaville, Congo For centuries, various pathologies have been treated with traditional medicines derived from medicinal plants. Such remedies must meet three requirements: safety, therapeutic efficacy, and quality. The objective of this study was to assess the anti-ulcer effect, microbiological and physicochemical quality, and phytochemical profile of a revealed medicinal recipe (RMR). Two models of gastroduodenal ulcer induction using two ulcerogenic agents were employed to evaluate the anti-ulcer effect in laboratory rodents. Classical methods were applied for microbiological and physicochemical quality assessment, following standardized AFNOR procedures, while phytochemical profiling was carried out using tube coloration reactions. The results showed that the recipe protects the gastric mucosa against ulcers induced by hydrochloric acid¨Cethanol mixture and ethanol in the presence of diclofenac. The recipe was free of pathogenic flora (Salmonella and Staphylococcus aureus) as well as total and fecal coliforms. The contamination levels of total aerobic mesophilic flora were 2.2×106 CFU/mL and 104 CFU/mL for yeasts and molds, compared with the standards of 103 and 102 CFU/mL, respectively. Moreover, the pH, electrical conductivity, total dissolved solids, and solvent content were 4.61, 3.625 mS/cm, 1835 ppm, and 20.04%, respectively. Phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, anthraquinones, oses and holosides, mucilages, reducing sugars, and the absence of saponosides. In conclusion, the revealed medicinal recipe is a mucosal protector of good microbiological and physicochemical quality and is rich in secondary metabolites. https://pubs.sciepub.com/ajmsm/13/4/6/ajmsm-13-4-6.pdf Ulcers microbiological quality phytochemistry revealed medicinal recipe