@article{ajmr20251353,
author={{Constant, Z¨¦br¨¦ Arthur and Moussa, Gbogbo and Thibaut, Ouina Toualy Serge and Parfait, N¡¯zi N¡¯goran and Rapha?l, Momo Si¨¦ Prince and Athanase, Kouassi Kra and Ibrahim, Konat¨¦ and Nathalie, Connil and Cl¨¦ment, Kouassi Kouassi},
title={Environmental Reservoirs of <i>bla, qnr </i>and <i>mcr</i>-1 Resistance Genes in <i>Enterobacteriaceae</i> from Animal and Wastewater Sources in Daloa, C?te d¡¯Ivoire},
journal={American Journal of Microbiological Research},
volume={13},
number={5},
pages={117--123},
year={2025},
url={https://pubs.sciepub.com/ajmr/13/5/3},
issn={2328-4137},
abstract={The environmental spread of antibiotic resistance genes is an increasing public health concern, particularly in resource-limited countries where waste management systems are often inadequate. This study aimed to identify Enterobacteriaceae in different ecosystems of Daloa city (C?te d¡¯Ivoire) and to assess the occurrence of critical resistance genes, including <i>bla</i>-TEM, <i>bla</i>-SHV, <i>bla</i>-CTX-M, <i>qnr</i>A, <i>qnr</i>B, <i>qnr</i>S, and <i>mcr</i>-1. Forty samples were collected from poultry and pig feces, wastewater from gutters, and a water body receiving domestic waste. Bacteria were isolated on selective media, identified using the API 20E system, and subjected to multiplex PCR for resistance gene detection. A total of 60 isolates were recovered, primarily <i>Escherichia coli</i> (73.3%), followed by <i>Salmonella</i> spp. (13.3%), <i>Enterobacter cloacae</i> (8.3%), and <i>Klebsiella oxytoca</i> (5%). The most frequently detected genes were <i>qnr</i>B (30%) and <i>bla</i>-SHV (15%), whereas <i>mcr-</i>1 was not detected in any isolate. Analysis of gene distribution across environments revealed that quinolone resistance genes were generally more widespread and prevalent than ¦Â-lactam resistance genes. These findings underscore the role of environmental ecosystems in the dissemination of antibiotic resistance and highlight the urgent need to strengthen surveillance and waste management strategies.},
doi={10.12691/ajmr-13-5-3}
publisher={Science and Education Publishing}
}