eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2025-10-26 13 5 117 123 10.12691/ajmr-13-5-3 AJMR20251353 article Z¨¦br¨¦ Arthur Constant zebre.arthur@gmail.com 1 Gbogbo Moussa 1 Ouina Toualy Serge Thibaut 1 N¡¯zi N¡¯goran Parfait 1 Momo Si¨¦ Prince Rapha?l 1 Kouassi Kra Athanase 1 Konat¨¦ Ibrahim 1 Connil Nathalie 2 Kouassi Kouassi Cl¨¦ment 2 Laboratory of Agrovalorisation, Department of Biochemistry and Microbiology, Jean LOROUGNON GUEDE University, Daloa, C?te d¡¯Ivoire Research Unit ¨C Bacterial Communication and Anti-infectious Strategies (CBSA, UR4312), University of Rouen Normandy, Evreux, France The environmental spread of antibiotic resistance genes is an increasing public health concern, particularly in resource-limited countries where waste management systems are often inadequate. This study aimed to identify Enterobacteriaceae in different ecosystems of Daloa city (Côte d¡¯Ivoire) and to assess the occurrence of critical resistance genes, including bla-TEM, bla-SHV, bla-CTX-M, qnrA, qnrB, qnrS, and mcr-1. Forty samples were collected from poultry and pig feces, wastewater from gutters, and a water body receiving domestic waste. Bacteria were isolated on selective media, identified using the API 20E system, and subjected to multiplex PCR for resistance gene detection. A total of 60 isolates were recovered, primarily Escherichia coli (73.3%), followed by Salmonella spp. (13.3%), Enterobacter cloacae (8.3%), and Klebsiella oxytoca (5%). The most frequently detected genes were qnrB (30%) and bla-SHV (15%), whereas mcr-1 was not detected in any isolate. Analysis of gene distribution across environments revealed that quinolone resistance genes were generally more widespread and prevalent than ¦Â-lactam resistance genes. These findings underscore the role of environmental ecosystems in the dissemination of antibiotic resistance and highlight the urgent need to strengthen surveillance and waste management strategies. https://pubs.sciepub.com/ajmr/13/5/3/ajmr-13-5-3.pdf Antibiotic resistance genes ¦Â-lactamases Enterobacteriaceae Environmental reservoirs Quinolones