@article{ajmr20251352,
author={{Shaha, Shyam Sundar and Shadia, Shamima Nasrin and Islam, Md. Tajul and Biswas, Shuvo and Sill, Shawon Chandra and Shuvo, Biswas Subrahmonium and Alam, Md. Rezaul and Banu, Mst. Nasrin and Biswas, Sabuj and Ghosh, Partha Pratim and Akter, Taslima and Hossen, Md. Mosharraf and Nasir, Md. Al and Sarker, Himangsu},
title={Prevalence, <i>inv</i>A Gene Detection and Antimicrobial Resistance Profiles of <i>Salmonella</i> spp. Isolated from Milk, Beef and Chicken Meat},
journal={American Journal of Microbiological Research},
volume={13},
number={5},
pages={109--116},
year={2025},
url={https://pubs.sciepub.com/ajmr/13/5/2},
issn={2328-4137},
abstract={<i>Salmonella</i> is a major foodborne pathogen found in various environmental sources, with the rise of antibiotic resistance posing a growing public health concern. This study isolate, identify, and characterize <i>Salmonella</i> spp. from milk, beef, and chicken meat to determine antibiotic resistance patterns and assess public health implications. A total of 120 samples were collected from different markets of Savar and Gazipur Sadar of Bangladesh. Presumptive identification of <i>Salmonella</i> isolates was performed by culturing on selective media, Gram¡¯s staining and confirmed by polymerase chain reaction (PCR) targeting the genus-specific primer of <i>inv</i>A. Bivariate analysis to assess the strong and significant correlations between the pairs of any of two antibiotic-resistant <i>Salmonella</i> spp. On the basis of cultural characteristics, the overall occurrence of <i>Salmonella</i> spp. was 20% in milk, 10% in beef and 25% in chicken meat. Furthermore, amplification of <i>inv</i>A gene revealed the occurrence as 63.63%. Among then 62.5% in milk, 75% in beef and 60% in chicken meat, respectively. The antimicrobial resistance pattern was determined by disc diffusion method. Among the antibiotics used in this study highest level of sensitivity were shown in streptomycin 100%, imipenem 100% and meropenem 80% in milk samples whereas highly resistance obtained to oxytetracycline 66.66% in beef and 83.33% in chicken meat. Notably, 20%, 33.33% and 16.66% isolates of milk, beef, and chicken meat, respectively were multidrug-resistant. <i>Salmonella</i> contamination in raw milk, beef and chicken poses a public health risk in the study area. Proper hygiene measures, awareness among sellers and judicious control use of antibiotic at the farm level are crucial to reducing contamination and antibiotic resistance.},
doi={10.12691/ajmr-13-5-2}
publisher={Science and Education Publishing}
}