eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2025-10-19 13 5 109 116 10.12691/ajmr-13-5-2 AJMR20251352 article Shyam Sundar Shaha 1 Shamima Nasrin Shadia 2 Md. Tajul Islam 2 Shuvo Biswas 3 Shawon Chandra Sill 4 Biswas Subrahmonium Shuvo 5 Md. Rezaul Alam 6 Mst. Nasrin Banu 7 Sabuj Biswas 8 Partha Pratim Ghosh 9 Taslima Akter 10 Md. Mosharraf Hossen 10 Md. Al Nasir 11 Himangsu Sarker himangsusarker96@gmail.com 11 Department of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Savar, Dhaka-1344, Bangladesh Department of Pathology, Gonoshasthaya Samaj Vittik Medical College, Savar, Dhaka-1344, Bangladesh Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh Faculty of Veterinary Medicine, Jashore University of Science and Technology, Jashore-7408, Bangladesh Department of Microbiology, Gono Bishwabidyalay, Savar, Dhaka-1344, Bangladesh Department of Paraclinical Courses, Gono Bishwabidyalay, Savar, Dhaka-1344, Bangladesh Probe International Clinical Research Pvt Ltd, Dhaka-1207, Bangladesh Department of Biotechnology & Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh 0Department of Clinical Courses, Gono Bishwabidyalay, Savar, Dhaka-1344, Bangladesh 1Department of Pharmacy, University of Asia Pacific, Dhaka-1215, Bangladesh Salmonella is a major foodborne pathogen found in various environmental sources, with the rise of antibiotic resistance posing a growing public health concern. This study isolate, identify, and characterize Salmonella spp. from milk, beef, and chicken meat to determine antibiotic resistance patterns and assess public health implications. A total of 120 samples were collected from different markets of Savar and Gazipur Sadar of Bangladesh. Presumptive identification of Salmonella isolates was performed by culturing on selective media, Gram¡¯s staining and confirmed by polymerase chain reaction (PCR) targeting the genus-specific primer of invA. Bivariate analysis to assess the strong and significant correlations between the pairs of any of two antibiotic-resistant Salmonella spp. On the basis of cultural characteristics, the overall occurrence of Salmonella spp. was 20% in milk, 10% in beef and 25% in chicken meat. Furthermore, amplification of invA gene revealed the occurrence as 63.63%. Among then 62.5% in milk, 75% in beef and 60% in chicken meat, respectively. The antimicrobial resistance pattern was determined by disc diffusion method. Among the antibiotics used in this study highest level of sensitivity were shown in streptomycin 100%, imipenem 100% and meropenem 80% in milk samples whereas highly resistance obtained to oxytetracycline 66.66% in beef and 83.33% in chicken meat. Notably, 20%, 33.33% and 16.66% isolates of milk, beef, and chicken meat, respectively were multidrug-resistant. Salmonella contamination in raw milk, beef and chicken poses a public health risk in the study area. Proper hygiene measures, awareness among sellers and judicious control use of antibiotic at the farm level are crucial to reducing contamination and antibiotic resistance. https://pubs.sciepub.com/ajmr/13/5/2/ajmr-13-5-2.pdf Antimicrobial resistance invA gene MDR prevalence Salmonella