eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2024-07-23 12 3 63 78 10.12691/ajmr-12-3-4 AJMR20241234 article Applications of Cyclodextrin-gluconotransferase in the Biosynthesis of Cyclodextrins: Characteristics, Sources and Production T¨¦nor Dias-Mendel Allode 1 Alode Cyrille Vodounon tenorallode8@gmail.com 1 Atindehou Gabin Dossou 1 No?l Christi Honzounnon 2 Akodji D¨¨fognon Fiacre Marcos Migan 2 3 Wilfried houenoukpo Hlouedje 4 Laboratory of Natural Sciences and Applications (LSNA), Higher Normal School of Natitingou, National University of Sciences, Technologies, Engineering, and Mathematics of Abomey, BP 72 Natitingou, Benin Research Unit on Non-Communicable Diseases and Cancer (UR-MNTC), Applied Biology Research Laboratory (LARBA), Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01BP 2009 Cotonou, Benin Experimental and Clinical Biology Unit (UBEC), Medical and Pharmaceutical Biotechnology Research Laboratory (LaRBiMeP), National Higher School of Applied Biosciences and Biotechnology of Dassa-Zoum¨¦ (ENSBBA), National University of Sciences, Technologies, Engineering and Mathematics of Abomey Cyclodextrin-gluconotransferases are microbial enzymes belonging to the ¦Á-amylase family. They synthesize cyclic oligosaccharides called cyclodextrins from starch substrates. The production of cyclodextrin-glucosyltransferases is generally carried out by fermentation in a liquid or solid medium. The review article aims to provide a comprehensive and in-depth overview of cyclodextrin-glucosyltransferases, highlighting their characteristics, sources, production, and applications in producing cyclodextrins. Websites such as ScienceDirect, NCBI, researchgate.net, scholar.google, core. Ac. uk, serve as a search engine for documents related to our review article. This article provides the answers to the in-depth understanding of cyclodextrin-gluconotransferases, production methods, industrial applications, and the diversity of strains that produce them. https://pubs.sciepub.com/ajmr/12/3/4/ajmr-12-3-4.pdf applications sources production cyclodextrin bacteria Cyclodextrin-gluconotransferase