eng Science and Education Publishing American Journal of Microbiological Research 2328-4137 2024-06-27 12 3 45 50 10.12691/ajmr-12-3-2 AJMR20241232 article Grace Kemsol Miedjim gracekemsolmied@yahoo.fr 1 2 Gbonon Mbengue Val¨¦rie 2 Kouam¨¦ Clarisse 2 Akaffou Adja Evelyne 3 Nd?h Ngrab¨¦ Nodje-Assal 4 Assohoun Egomli Stanislas 4 5 Marcelle Money Ettien 5 Franck Dj¨¦da Gnahor¨¦ 5 6 Kouakou Diely Anita Christelle 6 Ann¨¦ J.Claude 6 Kangah Tatiana 6 Ouattara Mohamed Baguy 6 Koffi St¨¦phane 6 Dosso Mireille 6 6 Institut Pasteur de C?te d¡¯Ivoire H?pital M¨¨re Enfant Bingerville CHU la Renaissance of Chad Introduction: The intestinal microbiota (IM) comprises microorganisms such as bacteria, viruses, and fungi that protect and live in symbiosis with the host. This article describes the cultivable bacterial population and their interactions, analyzing the variables of IM establishment in infants from birth (D0) to 3 months at the Bingerville Pediatric Hospital. Method: A 4-month longitudinal and prospective cohort study was conducted to perform a conventional bacteriological analysis and antibiotic susceptibility testing, searching for resistance markers from stool samples preserved at -80°C. Results: 52 infants were recruited for the initial sampling at D0, and 31 samples were collected at M3. At D0, only bifidobacteria were identified. At M3, bifidobacteria represented 44% of the isolated microorganisms, followed by E. faecalis, S. aureus, E. coli, Streptococcus sp, and yeasts. Conclusion: Bifidobacteria, E. faecalis, Streptococcus sp, E. coli, S. aureus, and yeasts constitute the intestinal microbiota of infants up to 3 months. Increasing the sample size and utilizing high-throughput sequencing or metagenomic methods are recommended for a better understanding of the intestinal microbiota. https://pubs.sciepub.com/ajmr/12/3/2/ajmr-12-3-2.pdf intestinal microbiota infants 0 to 3 months Mother and Child Hospital