eng Science and Education Publishing American Journal of Infectious Diseases and Microbiology 2328-4064 2024-07-01 12 3 52 59 10.12691/ajidm-12-3-2 AJIDM20241232 article Oumarou Ou¨¦draogo ouedoumarou.77@gmail.com 1 Dinanib¨¨ Kambir¨¦ 1 Issiaka Soulama 1 Edith C. Bougouma 2 Blami Kote 3 Luisa Nunziangeli 4 Henri Gautier Ou¨¦draogo 4 Yves Traor¨¦ 5 Serge Diagbouga 5 Seni Kouanda 5 Valentina Mangano 6 David Modiano 6 Giampietro Corradin 7 Sodiomon B. Sirima 7 Roberta Spaccapelo 8 Issa N¨¦bi¨¦ 8 D¨¦partement Biom¨¦dical et Sant¨¦ Publique, Institut de Recherche en Sciences de la Sant¨¦, Ouagadougou 03 BP 7047, Burkina Faso D¨¦partement Sant¨¦ publique et Sciences Biom¨¦dicales, Groupe de Recherche Action en Sant¨¦, Ouagadougou 06 BP 10248, Burkina Faso Departement de Math¨¦matiques et Informatique, Universit¨¦ de Ouahigouya, Ouahigouya 01 BP 346, Burkina Faso Polo d'Innovazione di Genomica, Genetica e Biologia, V. Mazzieri, 3, 05100 Terni, Italy D¨¦partement de Biochimie-Microbiologie, Laboratoire de Biochimie et d¡¯Immunologie Appliqu¨¦es, Universit¨¦ Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso Dipartimento di Sanit¨¤ Pubblica e Malattie Infettive, Universit¨¤ ¡°La Sapienza¡±, P.le Aldo Moro 5, 00185 Roma, Italy Biochemistry Department, University of Lausanne, 1066 Epalinges, Switzerland Dipartimento di Medicina Sperimentale, Universita Degli Studi Di Perugia, 06132 Perugia, Italy Antibody immune response play key role in the naturally acquired immunity to malaria. However, due to the complex life cycle and Plasmodium antigen diversity, there still is a need to search for protective antigens. We have used multiplex protein microarrays of 92 novel Plasmodium. falciparum alpha-helical coiled coil protein motifs to screen plasma samples obtained from children and adults belonging to three sympatric ethnic groups from malaria endemic area in Burkina Faso. Samples collected during a cross- sectional survey allowed to draw the IgG antibody response profile related to concurrent parasitaemia and clinical status at baseline. Samples collected from children under 5 years old who received longitudinal follow-up allowed to identify protective malaria antigens. The study results showed that the concurrent parasitemia influenced the antibody response to Plasmodium falciparum. The number of recognized antigens and the IgG antibody intensity were higher in uninfected volunteers compared to the infected people. The number of recognized antigens and the IgG antibody level were higher in asymptomatic individuals at baseline compared to symptomatic volunteers. The study results have shown that the IgG antibody response to six antigens (LR150B, LR163, MR232, MR259C, MR261A and MR282) were associated to protection against clinical malaria in children under five years old. https://pubs.sciepub.com/ajidm/12/3/2/ajidm-12-3-2.pdf malaria Plasmodium falciparum microarrays Burkina Faso