eng Science and Education Publishing American Journal of Biomedical Research 2014-11-12 2 4 67 69 10.12691/ajbr-2-4-2 AJBR2014242 article T Sudhakar 1 Sabitha Kandi 2 B venugopal 2 K. Bhagwan Reddy 3 Md. Rafi 3 Raj kumar 3 K. V. Ramana ramana_20021@rediffmail.com 4 Department of Biochemistry, HOD lab in-charge, Apollo Reach Hospital, Karimnagar, Andhra Pradesh, India Department of Biochemistry, Chalmeda Anadrao Institute of Medical Sciences, Karimnagar, India Department of Biochemistry, Prathima Institute of Medical Sciences, Karimnagar, India Department of Microbiology, Prathima Institute of Medical Sciences, Karimnagar, India The study was conducted to evaluate the differences in results obtained for assays of ionized calcium (iCa+2) by plain and heparinised blood sample and observe for any errors in values done by ion selective electrode (ISE) method and to determine which of the collection methods could be ideal and reliable. 49 samples of heparinised and 31 plain blood samples were analyzed at lab services, Apollo Reach hospital, Karimnagar, Telangana state for iCa+2 by ISE method using radiometer analyzer and the differences in data were documented statistically by calculating the mean and SD. The results of the study showed statistically significant difference in values of iCa+2 when blood was collected in plain tube (4.7±0.2) and with heparinised collection (4.4±0.3). It appears in the study that plain tube collection for the assay is ideal. http://pubs.sciepub.com/ajbr/2/4/2/ajbr-2-4-2.pdf iCa+2 calcium-neutralized lithium zinc heparin (CNLZ) heparinised and plain tube for iCa+2 iCa+2by ion selective electrode (ISE) method