Using L. rhamnosus as an Edible and Safe Agent to Detoxify and De-pathogenicity of B. cereus in Newborn Diets

Ashraf Albrakati^1,

¹Department of human anatomy, College of Medicine, Taif University, P.O. 11099, Taif 21944, Saudi Arabia

Cite this paper:
Ashraf Albrakati. Using L. rhamnosus as an Edible and Safe Agent to Detoxify and De-pathogenicity of B. cereus in Newborn Diets. Journal of Food and Nutrition Research. 2024; 12(10):409-419. doi: 10.12691/jfnr-12-10-2

Abstract

Infant meals are approved as a primary source of nutrition for babies, thus they should be nutrient-dense and come from a variety of sources. The current study seeks to assess the hygiene properties of infant foods in the cities of Taif and Makkah in order to improve their quality to satisfy industry standards. In Makkah and Taif, Saudi Arabia, 300 infant food samples were obtained at random from supermarkets and pharmacies. Microbiological examinations were done on the obtained samples. Also, molecular techniques were applied to confirm B. cereus pathogenicity. Probiotic bacteria were tested of their anti-microbial activity, and then L. rhamnosus as anti-B. cereus was added to infant diets and evaluated its activity as detoxifier using mice model. In almost all of the samples analyzed, total bacterial counts were found, with mean complete viable counts ranging from 1.586 to 3.543 log10 cfu/g. In addition, 196 Bacillus colonies were noted, with 27% of them including B. cereus. Despite being a rare species, B. cereus is the most deadly strain due to its pathogenicity and synthesis of severe toxins. As a result, the high effect of L. rhamnosus as anti-B. cereus led to its usage for detoxification and de-pathogenicity of the target pathogen, and their linked genes to enterotoxins were genetically detected. To validate that, L. rhamnosus was mixed with B. cereus and/or its emetic toxins and fed to mice as a food sample. The findings of this study demonstrated that L. rhamnosus reduced the toxicity of B. cereus and its enterotoxins, paving the way for this probiotic strain to be used as an edible and safe agent to protect infant's meals from hazardous microbial elements.

Keywords:
infant foods bacillus cereus Enterotoxins L. rhamnosus detoxification

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