Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2022, 10(5), 366-376
DOI: 10.12691/jfnr-10-5-5
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Quinoa (Chenopodium quinoa) Methionine Sulfoxide Reductase MSRA5.1 Interacts with Glutathione Synthase 2 to Improve Osmotic Stress Resistance in Arabidopsis Thaliana

Xiaofen Li1, 2, Hafeez Noor1, 2, Kaiyuan Cui1, 2, Xiangyun Wu3, Pengcheng Ding1, 2, Min Sun1, 2, Yongkang Ren1, 2, and Zhiqiang Gao1, 2

1College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China

2Ministerial and Provincial Co-Innovation Centre for Endemic Crops Production with High-quality and Effciency in Loess Plateau, Taigu 030801, China, Shanxi Agricultural University, Shanxi, Taiyuan 030031, China

3Shanxi Jiaqi Agri-Tech Co., Ltd., Taiyuan 030006, China

Pub. Date: May 18, 2022

Cite this paper:
Xiaofen Li, Hafeez Noor, Kaiyuan Cui, Xiangyun Wu, Pengcheng Ding, Min Sun, Yongkang Ren and Zhiqiang Gao. Quinoa (Chenopodium quinoa) Methionine Sulfoxide Reductase MSRA5.1 Interacts with Glutathione Synthase 2 to Improve Osmotic Stress Resistance in Arabidopsis Thaliana. Journal of Food and Nutrition Research. 2022; 10(5):366-376. doi: 10.12691/jfnr-10-5-5

Abstract

Reactive oxygen species (ROS) produced by abiotic stress in plant cells can lead to the oxidation of methionine (Met). Met sulfoxide reductases (MSRs) reduce oxidized Met and protect plants from oxidative damage. However, the function of the MSR gene family in quinoa remains unknown. In the present study, a set of eight full-length Chenopodium quinoa MSR (CqMSR) complementary DNAs was isolated. And their responses to salt, drought, heat and cold stress were analyzed. To the best of our knowledge, no information on function and substrates of CqMSRA5.1 has been reported in quinoa. In the present study, its constitutive overexpression in Arabidopsis (AtOE) resulted in notable increase in MSR activity, and enhanced the tolerance of seedlings to osmotic stress. The effect of active CqMSRA5.1 in Arabidopsis decreased ROS accumulation. Quinoa glutamine synthetase 2 (GSH2) a potential interaction substrate of CqMSRA5.1, was obtained by screening STRING database, further confirmed by means of yeast two-hybrid, and bimolecular fluorescence complementation assay. Under osmotic stress, the expression of AtGSH2 and the total content of GSH in AtOE lines increased significantly, and the ratio of GSH/GSSG decreased significantly compared to wild type. BSO, one inhibitor of GST, can partially impair the phenotype of the CqMSRA5.1-induced response to osmotic stress. Together, CqMSRA5.1 participates in osmotic response partially via CqGSH2.

Keywords:
Chenopodium quinoa Methionine sulfoxide reductase Abiotic stress Reactive oxygen species CqMSRA5.1

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